Increased proteolytic susceptibility of carboxypeptidase Y caused by modification of the disulfide zipper.
نویسندگان
چکیده
To investigate the structural importance of a "disulfide zipper" motif of carboxypeptidase Y, disulfide-deficient mutant enzymes were expressed in two strains of Saccharomyces cerevisiae. The mutant enzymes were rapidly degraded into fragments by intracellular proteases. Thus, it is concluded that the disulfide zipper is essential in maintaining the structural integrity of CPase Y against proteolytic susceptibility.
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عنوان ژورنال:
- Bioscience, biotechnology, and biochemistry
دوره 66 6 شماره
صفحات -
تاریخ انتشار 2002